Sites of Biosynthesis of Outer and Inner Membrane Proteins of Neurospora crassa Mitochondria

Outer and inner membranes of Neurospora crassa mitochondria were separated by the combined swelling, shrinking, sonication procedure. Membranes were characterized by electron microscopy and by marker enzyme activities. A red carotenoid pigment was found to be concentrated in the outer membrane. The inner mitochondrial membrane was resolved into about 20 protein bands on polyacrylamide gel electrophoresis, whereas the outer membrane shows essentially one single protein band. Only negligible incorporation of radioactive amino acids occurs into outer membrane when isolated mitochondria are synthesizing polypeptide chains. In agreement with this observation labeling of outer membrane protein is almost entirely blocked, when whole Neurospora cells are incubated with radioactive amino acids in the presence of cycloheximide, an inhibitor of cytoplasmic protein synthesis. Finally, the essential electrophoretic protein band from outer membrane does not become labeled when mitochondria are incubated with radioactive amino acids either in vitro or in vivo in the presence of cycloheximide. It is concluded that the vast majority, if not all, of the outer membrane protein is synthesized by the cytoplasmic system and that polypeptide chains formed by the mitochondrial ribosomes are integrated into the inner mitochondrial membrane

Turnover of outer and inner membrane proteins of rat liver mitochondria

Recent reports on the properties and composition of outer and inner mitochondrial membranes suggest that outer and inner membranes of mitochondria are derived from different sources of the cell [l-3]. I

Clinical surveillance of thrombotic microangiopathies in Scotland, 2003-2005

The prevalence, incidence and outcomes of haemolytic uraemic syndrome (HUS) and thrombotic thrombocytopaenic purpura (TTP) are not well established in adults or children from prospective studies. We sought to identify both outcomes and current management strategies using prospective, national surveillance of HUS and TTP, from 2003 to 2005 inclusive. We also investigated the links between these disorders and factors implicated in the aetiology of HUS and TTP including infections, chemotherapy, and immunosuppression. Most cases of HUS were caused by verocytotoxin-producing Escherichia coli (VTEC), of which serotype O157 predominated, although other serotypes were identified. The list of predisposing factors for TTP was more varied although use of immunosuppressive agents and severe sepsis, were the most frequent precipitants. The study demonstrates that while differentiating between HUS and TTP is sometimes difficult, in most cases the two syndromes have quite different predisposing factors and clinical parameters, enabling clinical and epidemiological profiling for these disorders

Spiral strand cables subjected to high velocity fragment impact

Structural cables are widely adopted around the world in offshore construction, sports stadia, large scale bridges, Ferris wheels and suspended canopy and fabric structures. However, the robustness of such structures to blast or impact is uncertain with a particular concern related to the loss of a primary structural cable when damaged by high velocity blast fragmentation. This paper presents the first ever numerical and experimental study on commonly used high-strength steel spiral strand cables subjected to high velocity fragment impact. Spiral strand cables were impacted by 20 mm fragment simulating projectiles travelling at velocities between 200 and 1400 m/s. Complex 3D non-linear finite element models were developed and carefully compared with experimental tests. The penetration resistance of the cables and resultant damage were studied with respect to fragment impact velocity. It was found that for all the impact velocities, the fragment penetration depth was less than half of the cable diameter demonstrating a considerable amount of resilience. Considering the damage caused, the residual cable breaking strengths were estimated and found to be still higher than the minimum breaking load of an un-damaged cable. The numerical models were also able to reproduce the main features of the impact tests, including the extent of localised damage area, the fragment penetration depth and mode of individual wire failures, thus demonstrating their potential to be widely used in industry for structural resilience and robustness assessments by structural engineers

Transverse Demagnetization Dynamics of a Unitary Fermi Gas

Understanding the quantum dynamics of strongly interacting fermions is a problem relevant to diverse forms of matter, including high-temperature superconductors, neutron stars, and quark-gluon plasma. An appealing benchmark is offered by cold atomic gases in the unitary limit of strong interactions. Here we study the dynamics of a transversely magnetized unitary Fermi gas in an inhomogeneous magnetic field. We observe the demagnetization of the gas, caused by diffusive spin transport. At low temperatures, the diffusion constant saturates to the conjectured quantum-mechanical lower bound $\simeq \hbar/m$, where $m$ is the particle mass. The development of pair correlations, indicating the transformation of the initially non-interacting gas towards a unitary spin mixture, is observed by measuring Tan's contact parameter.Comment: 8 pages, 6 figures. Accepted versio

Excitation spectrum of a two-component Bose-Einstein condensate in a ring potential

A mixture of two distinguishable Bose-Einstein condensates confined in a ring potential has numerous interesting properties under rotational and solitary-wave excitation. The lowest-energy states for a fixed angular momentum coincide with a family of solitary-wave solutions. In the limit of weak interactions, exact diagonalization of the many-body Hamiltonian is possible and permits evaluation of the complete excitation spectrum of the system.Comment: 4 pages, 1 figur

Optical properties of high quality Cu2ZnSnSe4 thin films

Cu2ZnSnSe4 thin films, fabricated on bare or molybdenum coated glass substrates by magnetron sputtering and selenisation, were studied by a range of techniques. Photoluminescence spectra reveal an excitonic peak and two phonon replicas of a donor-acceptor pair (DAP) recombination. Its acceptor and donor ionisation energies are 27 and 7 meV, respectively. This demonstrates that high-quality Cu2ZnSnSe4 thin films can be fabricated. An experimental value for the longitudinal optical phonon energy of 28 meV was estimated. The band gap energy of 1.01 eV at room temperature was determined using optical absorption spectr

Coherent Behavior and Nonmagnetic Impurity Effects of the Spin Disordered State in NiGa2_2S4_4

Nonmagnetic impurity effects of the spin disordered state in the triangular antiferromagnet NiGa$_2$S$_4$ was studied through magnetic and thermal measurements for Ni$_{1-x}$Zn$_x$Ga$_2$S$_4$ (0.0\le x\le 0.3). Only 1 % substitution is enough to strongly suppress the coherence observed in the spin disordered state. However, the suppression is not complete and the robust feature of the T^2 dependent specific heat and its scaling behavior with the Weiss temperature indicate the existence of a coherent Nambu-Goldstone mode. Absence of either conventional magnetic order or bulk spin freezing suggests a novel symmetry breaking of the ground state.Comment: 4 pages, 4 figure

Orchard Day, August 18, 1955; Results of Fruit Research

Storing peaches / Donald Comin -- Trends in storages / Donald Comin -- Grape varieties ; Grape soil management and fertilization ; Nitrogen fertilizers for apple trees / J. M. Beattie -- Colorset on Golden Delicious and Grimes ; Planting distance influence on yield of apples ; Harvest dates for apples ; Summer varieties of apples ; Pre-harvest spraying for Baldwin / C. W. Ellenwood -- Peach investigations ; The blueberry planting ; Strawberry studies ; Plum varieties and culture ; Grape weed control studies / R. G. Hill, Jr. -- Semi-dwarf apple trees in Ohio / Freeman S. Howlett and T. E. Fowler -- The Franklin Apple / Freeman S. Howlett and C. W. Ellenwood -- Status of intermediate (trunk-forming) stocks for the apple ; Pruning the newly planted apple tree ; Status of the pear industry in Ohio / Freeman S. Howlett -- Pear variety collection indicates several deserve limited trial / Freeman S. Howlett and C. W. Ellenwoo